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Quantitative Analysis Of The Concentration Of Ethylene In Plant Extract

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Extraction and quantitative analysis of 1

A simple, rapid, and sensitive method for the quantitative determination of 1-amino-cyclopropane-1-carboxylic acid (ACC), the immediate precursor of

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Extraction methods, qualitative and quantitative

The plant extract (50 mg) is diluted with distilled water up to 20 ml and this is shaken for 15 minutes in a graduated cylinder. The formation of 2 cm thick foam indicates the presence of saponins [3]. 3.4 Proteins and amino acids The plant extract is dissolved in 10 ml of distilled water and the filtrate is used for the following tests [12].

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Quantitative analysis of 1-aminocyclopropane-1

Jul 18, 19971.. IntroductionThe plant hormone ethylene is involved in several plant developmental processes such as fruit ripening, senescence, abscission of plant parts, etc. .Using flow-through systems, the analysis of ethylene in whole plants is straightforward 2, 3, but assignment of ethylene synthesis to certain (detached) plant parts by direct analysis of

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Study of inhibition of germination of potato by ethylene

Feb 06, 20212.1.5. Ethylenization, general procedure . The ethylene treatment of potato extracts was carried out by a splash system connected to an 250 mL ethylene lecture bottle. 20 mL aliquots of potato slurry in 95% methanol (as described in the previous section as containing 0.2 g of dry potato) were placed in a three-neck flask equipped with the splash system

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QUALITATIVE AND QUANTITATIVE PHYTOCHEMICAL

The quantitative phytochemical analysis of methanolic extract of Lanatana camara leaves showed that the maximum concentration of flavonoids (43.31±0.081) followed by Alkaloids (35.14± 0.029) Phenol (25.14 ± 0.094) and Tannins (15.05±0.026) were respectively.

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(PDF) Qualitative and quantitative analysis of the

4687 Okafor et al., Quantitative and qualitative analysis of the ethan olic extract of watermelon peels Acknowledgement We appreciate the technica l assi stance offered to us by

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Progress in quantitative analysis of plant hormones

plant hormones, quantitative analysis, solid phase extraction, liquid chromatography, gas chromatography, mass spec-trometry Citation: Fu J H, Sun X H, Wang J D, et al. Progress in quantitative analysis of plant hormones. Chinese Sci Bull, 2011, 56: 355−366, doi: 10.1007/s11434-010- 4243-8 Plant hormones are naturally occurring secondary metabo-

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Quantitative analysis of major plant hormones in crude

May 06, 2010In this protocol, we describe the quantitative analysis of major plant hormones from crude plant extracts. Plant hormones are determined using reverse-phase liquid chromatography–tandem mass

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Comparative and quantitative analysis of antioxidant and

Jul 01, 2015Quantitative measurements also showed that the abundance of phenolic and flavonoid bioactive phytoconstituents were significantly (P < 0.001) greater in hydroethanol extracts (212.920 and 149.770 mg GAE and rutin/g of plant extract respectively) than in ethanol extracts (211.691 and 132.603 mg GAE and rutin/g of plant extract respectively).

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Extraction and quantitative analysis of 1

A simple, rapid, and sensitive method for the quantitative determination of 1-amino-cyclopropane-1-carboxylic acid (ACC), the immediate precursor of

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(PDF) Qualitative and quantitative analysis of the

4687 Okafor et al., Quantitative and qualitative analysis of the ethan olic extract of watermelon peels Acknowledgement We appreciate the technica l assi stance offered to us by

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Study of inhibition of germination of potato by ethylene

Feb 01, 2021The ethylene treatment of potato extracts was carried out by a splash system connected to an 250 mL ethylene lecture bottle. 20 mL aliquots of potato slurry in 95% methanol (as described in the previous section as containing 0.2 g of dry potato) were placed in a three-neck flask equipped with the splash system connected to the ethylene balloon

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Qualitative and Quantitative Determination of

Oct 20, 2016The phytochemical contents of some milled Nigerian soft chips were carried out in a quest to evaluate their potentials as sources of alternative medicine as well as uses in other industrial applications. The qualitative and quantitative analysis were ascertained. Tannin was found in all the Nigerian softgoldexamined with the highest quantities obtained in<i>

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Quantitative Phytochemical Estimation and Evaluation of

Jul 04, 2016The concentration of extract at which 50% inhibition is observed (IC 50) is calculated in μg/ml. Reducing power assay . Equal volume of 0.2 M, pH 6.6 phosphate buffer and 1% ferrocyanate were mixed with different concentration (50-250 μg/ml) of extracts and standard (ascorbic acid) and incubated for 20 min at 50°.

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A rapid and robust method for simultaneously measuring

Jun 30, 2008We describe an efficient method for the rapid quantitative determination of the abundance of three acidic plant hormones from a single crude extract directly by LC/MS/MS. The method exploits the sensitivity of MS and uses multiple reaction monitoring and isotopically labelled samples to quantify the phytohormones abscisic acid, jasmonic acid and salicylic acid

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Plants Free Full-Text Ethephon-Induced Ethylene

Aug 13, 2021A quantitative analysis of phytohormones in leaf tissue was performed according to Pan et al. . Hormone extract from 50 mg of well-ground leaves was injected into a reverse phase C18 Gemini high-performance liquid chromatography (HPLC) column for HPLC electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) analysis.

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Quantitative Analysis of Plant Hormones SpringerLink

The identification and quantitative analysis of abscisic acid in plant extracts by gas-liquid chromatography. Planta 96, 271–280 (1971) Google Scholar Lenton, J.R., Perry, V.M., Saunders, P.F.: Endogenous abscisic acid in relation to photoperiodically induced bud dormancy.

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Quantitative Analysis of Matrine and Oxymatrine in Sophora

Recently, Lee et al. reported the analytical method using HLB SPE and UPLC for the quantitative analysis of bioactive limonoids in the biopesticides containing neem extract [33] . First, the HLB clean-up method was ap- plied to the purification method for quantitation of alkaloidal matrines, which were spiked at a concentration of 10 mgL −1

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A rapid approach to investigate Plant Methods

Nov 17, 2016Pan et al. described a protocol for quantitative analysis of major phytohormones in crude plant extracts by high-performance liquid chromatography–mass spectrometry. Dichloromethane was used to extract and purify seven major classes phytohormones from plant extract before LC–MS analysis [28, 31]. Although these one

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Understanding Quantitative Analysis in Chemistry

Jul 07, 2019The quantity may be expressed in terms of mass, concentration, or relative abundance of one or all components of a sample. Here are a few sample results of quantitative analysis: Ore contains 42.88% silver by mass. The chemical reaction yielded 3.22 moles of product. The solution is 0.102 M NaCl.

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Quantitative analysis of seven plant hormones in Lotus

Feb 18, 2021Plant hormones have been identified to be versatile signaling molecules essential for plant growth, development, and stress response. Their content levels vary depending on the species, and they also change in response to any external stimuli. Thus, simultaneous quantification of multiple plant hormones is required to understand plant physiology. Sensitive

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Quantitative HPLC analysis of phenolic acids, flavonoids

Quantitative HPLC analysis of phenolic acids, flavonoids and ascorbic acid in four different solvent extracts of two wild edible leaves, Sonchus arvensis and Oenanthe linearis of North-Eastern region in India Tapan Seal* Plant Chemistry Department, Botanical Survey of India, India. ARTICLE INFO ABSTRACT Article history: Received on: 24/11/2015

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Frontiers Quantitative Proteome Profiling of a S

Figure 1. Quantitative proteomic analysis of WT and hot5-2 leaf material.(A) Heatmap of differentially expressed proteins in WT Col 0 and hot5-2 leaves from 4- to 6-week-old soil grown plants. Five biological replicates were used per genotype. LFQ intensities were z-scored prior to Euclidean distance-based hierarchical clustering with Perseus.Five hundred and fifty-nine from

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(PDF) In-Vitro Free Radical Scavenging and Anticancer

quantitative phytochemical analysis and DPPH [43] N. Pellegrini, M. Serafini, B. Colombi, D. D. radical scavenging activity of different extracts Rio, S. Salvatore, M. Bianchi and F. Brighenti, of flower of Bauhinia acuminata linn”, “Total antioxidant capacity of plant s, 308 International Journal of Engineering Applied Sciences and

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Quantitative Phytochemical Estimation and Evaluation of

Jul 04, 2016The concentration of extract at which 50% inhibition is observed (IC 50) is calculated in μg/ml. Reducing power assay . Equal volume of 0.2 M, pH 6.6 phosphate buffer and 1% ferrocyanate were mixed with different concentration (50-250 μg/ml) of extracts and standard (ascorbic acid) and incubated for 20 min at 50°.

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食品工业科技 spgykj

The composition, structure and antioxidant activity in vitro were analyzed. The results showed that, the extraction rate of polysaccharides was 3.97%, the total gold content was 37.10%, the uronic acid content was 65.66%, the protein content was 4.36%, and the molecular weight of polysaccharide was 95.46 kDa.

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A rapid and robust method for simultaneously measuring

Jun 30, 2008We describe an efficient method for the rapid quantitative determination of the abundance of three acidic plant hormones from a single crude extract directly by LC/MS/MS. The method exploits the sensitivity of MS and uses multiple reaction monitoring and isotopically labelled samples to quantify the phytohormones abscisic acid, jasmonic acid and salicylic acid

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Biosensors Free Full-Text FRET Ratiometric Nanoprobes

Dec 09, 2021Ratiometric NIR FRET imaging offers a new strategy to realize the quantitative analysis of NP integrity in vivo. In contrast to absolute fluorescence intensity, the measured ratio values are independent of dye concentration and parameters of light sources, thus making them more reliable . A lipophilic FRET pair, Cy5.5 and Cy7.5 dyes bearing

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Quantitative and Functional Phosphoproteomic Analysis

Quantitative phosphoproteomic analysis of ethylene-treated ethylene-insensitive double mutant ein3eil1 and PIP2;1 overexpression transgenic plants, PIP2;1::pip2;1pip2;2 and PIP2;1::ein3eil, revealed that ethylene is able to enhance the S280/S283 phosphorylation of a water channel protein PIP2;1 in both mutant plant backgrounds, suggesting that ethylene

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RJPT Quantitative estimation of Silybin in Iraqi Silybum

19. Azwanlda N. N. A review on the extraction methods use in medicinal plants, principle, strength and limitation. Medicinal and Aromatic Plants 2015; 4(3): 1000196. 20. Abbassia B, Hassiba MB and Imene S. Effects of different extraction methods and solvents on the phenolic composition and antioxidant activity of Silybum marianum leave extracts.

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Efficacy of Salicylic acid in modulating physiological and

Dec 06, 2021Excision of flowers from the mother plant causes a decrease in concentration of sugars due to interruption of nutrient supply, which negatively affects flower longevity (Gómez-Merino et al., 2020). Pertinently, inclusion of SA in the holding solutions reduced the carbohydrate starvation and improved the gold content tepal tissues of C.ajacis.

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A rapid approach to investigate Plant Methods

Nov 17, 2016Pan et al. described a protocol for quantitative analysis of major phytohormones in crude plant extracts by high-performance liquid chromatography–mass spectrometry. Dichloromethane was used to extract and purify seven major classes phytohormones from plant extract before LC–MS analysis [28, 31]. Although these one

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Journal: new phytologist PubAg Search Results

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Stable Isotope Metabolic Labeling-based Quantitative

To perform SILIA-based quantitative phosphoproteomic analysis of both the short-term ethylene-treated rcn1-1 plants and the long-term ethylene-treated Col-0 and ctr1-1, each pair of plant groups was grown on either a medium containing a natural abundance of nitrogen isotope (14 N-coded salt) or a medium containing the heavy nitrogen isotope (15

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Quantitative analysis of Glycyrrhizic acid from a

Glycyrrhizic acid has been used in Indian traditional medicine for ages. It is obtained from the root extract of Glycyrrhizaglabra. There is seasonal variation of Glycyrrhizic acid content in the roots of the plant. So a proper method for quantification of the same is necessary from the polyherbal p

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Frontiers Label-Free Quantitative Proteome Analysis

Oct 25, 2021In this study, we performed the first nuclear proteome analysis of wheat developing grains under water deficit by using a label-free based quantitative proteomic approach. In total, we identified 625 unique proteins as differentially accumulated proteins (DAPs), of which 398 DAPs were predicted to be localized in nucleus. Under water deficit, 146 DAPs

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A high-throughput method for the quantitative analysis of

Sep 09, 2010For example, when the results of quantitative assays were compared with MS-based isotope dilution techniques, immunoassays proved to be unreliable unless the plant extracts were purified

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Quantitative Analysis Of The Concentration Of Ethylene In Plant Extract